GAPDH
Gene Ontology Biological Process
- apoptotic process [IMP]
- carbohydrate metabolic process [IDA]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [IDA]
- glycolytic process [IDA]
- microtubule cytoskeleton organization [IDA]
- multicellular organismal development [ISO]
- negative regulation of translation [ISO]
- neuron apoptotic process [IDA, IMP]
- oxidation-reduction process [ISO]
- peptidyl-cysteine S-trans-nitrosylation [IDA]
- protein stabilization [IDA]
- response to ammonium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [ISO, ISS]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [IDA]
- mitochondrion [ISO]
- nuclear membrane [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
AKT1
Gene Ontology Biological Process
- activation-induced cell death of T cells [ISO]
- aging [IDA]
- apoptotic mitochondrial changes [ISO]
- cell projection organization [IDA]
- cellular response to epidermal growth factor stimulus [ISO]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [ISO]
- cellular response to growth factor stimulus [ISO]
- cellular response to hypoxia [IDA]
- cellular response to insulin stimulus [IMP, ISO]
- cellular response to mechanical stimulus [IDA]
- cellular response to organic cyclic compound [IDA]
- cellular response to peptide [ISO]
- execution phase of apoptosis [ISO]
- germ cell development [ISO]
- glucose homeostasis [ISO]
- glucose metabolic process [ISO]
- glucose transport [ISO]
- glycogen biosynthetic process [IMP]
- glycogen cell differentiation involved in embryonic placenta development [ISO]
- glycogen metabolic process [ISO]
- hyaluronan metabolic process [IEP]
- inflammatory response [ISO]
- insulin receptor signaling pathway [IMP, ISO, ISS]
- insulin-like growth factor receptor signaling pathway [ISO, ISS]
- intracellular signal transduction [ISO]
- labyrinthine layer blood vessel development [ISO]
- maternal placenta development [ISO]
- negative regulation of JNK cascade [IDA]
- negative regulation of apoptotic process [ISO, TAS]
- negative regulation of autophagy [ISO]
- negative regulation of cell size [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO, ISS]
- negative regulation of endopeptidase activity [ISO]
- negative regulation of fatty acid beta-oxidation [ISO]
- negative regulation of plasma membrane long-chain fatty acid transport [ISO]
- negative regulation of protein kinase activity [IDA, ISO]
- negative regulation of proteolysis [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO, ISS]
- osteoblast differentiation [ISO]
- peptidyl-serine phosphorylation [IDA, ISO]
- peripheral nervous system myelin maintenance [ISO]
- phosphorylation [ISO, ISS]
- positive regulation of apoptotic process [IMP]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of cell growth [IDA, ISO]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [ISO]
- positive regulation of endothelial cell proliferation [ISO, ISS]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of lipid biosynthetic process [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of sodium ion transport [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of vasoconstriction [IMP]
- protein catabolic process [ISO]
- protein import into nucleus, translocation [ISO]
- protein kinase B signaling [ISO]
- protein phosphorylation [IDA, ISO, ISS]
- protein ubiquitination [ISO]
- regulation of cell migration [IMP, ISO]
- regulation of glycogen biosynthetic process [ISO]
- regulation of myelination [ISO]
- regulation of neuron projection development [ISO, ISS]
- regulation of protein localization [IMP, ISO]
- response to UV-A [ISO]
- response to fluid shear stress [ISO]
- response to food [ISO]
- response to hormone [ISO]
- response to oxidative stress [ISS]
- signal transduction [IDA]
- striated muscle cell differentiation [ISO]
- translation [IMP]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- ATP binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [IDA, ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [IPI]
- protein serine/threonine kinase activity [IDA, ISO, ISS, TAS]
- protein serine/threonine/tyrosine kinase activity [ISO]
- 14-3-3 protein binding [ISO]
- ATP binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [IDA, ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [IPI]
- protein serine/threonine kinase activity [IDA, ISO, ISS, TAS]
- protein serine/threonine/tyrosine kinase activity [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Glyceraldehyde-3-phosphate dehydrogenase interacts with phosphorylated Akt resulting from increased blood glucose in rat cardiac muscle.
Here we describe the interaction of phosphorylated approximately 40 kDa protein with phosphorylated Akt which is a serine/threonine kinase resulting from increased blood glucose in rat cardiac muscle. Mass spectrometry analysis revealed that this protein was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, increase in Akt and GAPDH phosporylation and induction of their association were both observed after insulin stimulation in the H9c2 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AKT1 GAPDH | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| AKT1 GAPDH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID