BAIT

CTF4

CHL15, POB1, chromatin-binding protein CTF4, L000000326, YPR135W
Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
Saccharomyces cerevisiae (S288c)
PREY

POL12

DNA-directed DNA polymerase alpha subunit POL12, L000002698, YBL035C
B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Replisome function during replicative stress is modulated by histone h3 lysine 56 acetylation through ctf4.

Luciano P, Dehe PM, Audebert S, Geli V, Corda Y

Histone H3 lysine 56 acetylation in Saccharomyces cerevisiae is required for the maintenance of genome stability under normal conditions and upon DNA replication stress. Here we show that in the absence of H3 lysine 56 acetylation replisome components become deleterious when replication forks collapse at natural replication block sites. This lethality is not a direct consequence of chromatin assembly defects ... [more]

Genetics Apr. 01, 2015; 199(4);1047-63 [Pubmed: 25697176]

Throughput

  • Low Throughput

Additional Notes

  • Figure S7

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POL12 CTF4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High2BioGRID
3598226
CTF4 POL12
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2566BioGRID
2074805
CTF4 POL12
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID