BAIT

PHS1

enoyl-CoA hydratase PHS1, YJL097W

Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking

GO Process (3)

GO Function (2)

GO Component (6)

Gene Ontology Biological Process

fatty acid elongation [IMP]

sphingolipid biosynthetic process [IMP]

vacuolar transport [IMP]

Gene Ontology Molecular Function

3-hydroxyacyl-CoA dehydratase activity [IMP]
enoyl-CoA hydratase activity [IDA]

Gene Ontology Cellular Component

endoplasmic reticulum [IDA]

fungal-type vacuole [IDA]

fungal-type vacuole membrane [IDA]

integral component of endoplasmic reticulum membrane [IDA]

integral component of membrane [ISM]

nuclear outer membrane-endoplasmic reticulum membrane network [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ELO3

SUR4, APA1, SRE1, VBM1, fatty acid elongase ELO3, L000002245, YLR372W

Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids.

Obara K, Kojima R, Kihara A

Very long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and we identified the VPS21 gene, encoding a Rab GTPase, as such a ... [more]

J. Lipid Res. Mar. 01, 2013; 54(3);831-42 [Pubmed: 23325927]

Throughput

Low Throughput

Ontology Terms

vegetative growth (APO:0000106)
heat sensitivity (APO:0000147)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PHS1 ELO3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4428	BioGRID	1993205

Curated By

BioGRID

Interaction Summary