A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

MYB82 functions in regulation of trichome development in Arabidopsis.

Liang G, He H, Li Y, Ai Q, Yu D

Trichome initiation and patterning are controlled by the TTG1-bHLH-MYB regulatory complex. Several MYB transcription factors have been determined to function in trichome development via incorporation into this complex. This study examined the role of MYB82, an R2R3-MYB transcription factor, in Arabidopsis trichome development. MYB82 was revealed to be a nuclear-localized transcription activator. Suppression of MYB82 function by fusion with a ... [more]

J. Exp. Bot. Jul. 01, 2014; 65(12);3215-23 [Pubmed: 24803498]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MYB82 GL3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Liang G (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MYB82

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

GL3