An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Methylation-controlled J-protein MCJ acts in the import of proteins into human mitochondria.

Schusdziarra C, Blamowska M, Azem A, Hell K

Loss of expression of the methylation-controlled J gene, MCJ (DNAJC15), is observed in cases of several tumors and plays a crucial role in the chemoresistance of ovarian cancer cells. Aside from the pathophysiological effects, almost nothing is known about the cellular function of MCJ. Here, we provide the first evidence that MCJ acts in the biogenesis of mitochondria. Our results ... [more]

Hum. Mol. Genet. Apr. 01, 2013; 22(7);1348-57 [Pubmed: 23263864]

Throughput

Low Throughput

Additional Notes

Figure 3A

Curated By

BioGRID

Interaction Summary

TIMM17B

Gene Ontology Biological Process

Gene Ontology Cellular Component

TIMM50

Gene Ontology Biological Process

Gene Ontology Molecular Function

interleukin-2 receptor binding [IDA]phosphoprotein phosphatase activity [IDA]protein binding [IPI]protein serine/threonine phosphatase activity [IDA]protein tyrosine phosphatase activity [IDA]ribonucleoprotein complex binding [IDA]