An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

U box proteins as a new family of ubiquitin-protein ligases.

Hatakeyama S, Yada M, Matsumoto M, Ishida N, Nakayama KI

The U box is a domain of approximately 70 amino acids that is present in proteins from yeast to humans. The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin-protein ligase (E3) to catalyze ubiquitin chain formation on artificial substrates. E3 ... [more]

J. Biol. Chem. Aug. 31, 2001; 276(35);33111-20 [Pubmed: 11435423]

Throughput

Low Throughput

Additional Notes

E2 -UbcH5C
E3 auto-ubiquitination assay
Figure 6
rabbit E1

Curated By

BioGRID

Interaction Summary

UBE4A

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin protein ligase activity [IDA]ubiquitin-ubiquitin ligase activity [IDA]

Gene Ontology Cellular Component