BAIT
SUMO1
GMP1, PIC1, SENTRIN, SMT3, SMT3H3, SMTP3, SUMO-1, Smt3C, Ubl1
SMT3 suppressor of mif two 3 homolog 1 (yeast)
GO Process (14)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
- PML body organization [IMP]
- negative regulation of DNA binding [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- palate development [IMP]
- positive regulation of calcium-transporting ATPase activity [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein localization to nuclear pore [IMP]
- protein sumoylation [IDA, IMP, ISO]
- regulation of calcium ion transmembrane transport [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Mus musculus
PREY
PIAS3
Pias3l
protein inhibitor of activated STAT 3
GO Process (4)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
PIAS3 induces SUMO-1 modification and transcriptional repression of IRF-1.
Interferon regulatory factor-1 (IRF-1) is a transcriptional activator that is involved in interferon response, regulation of cell growth and oncogenesis. To try to identify the molecules that regulate the function of IRF-1, we performed yeast two-hybrid screening and isolated protein inhibitor of activated STAT3 (PIAS3) as an IRF-1-binding protein. This protein was also found to bind with small ubiquitin-related modifier-1 ... [more]
FEBS Lett. Oct. 23, 2002; 530(1);204-8 [Pubmed: 12387893]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Figure 2 - This could reflect a covalent interaction because this assay also reports covalent modifications with SUMOs
- Likely protein-protein interaction
Curated By
- BioGRID