BAIT
UBE2I
5830467E05Rik, F830028O17Rik, UBC9, Ubce2i, Ubce9
ubiquitin-conjugating enzyme E2I
GO Process (10)
GO Function (10)
GO Component (8)
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of intracellular steroid hormone receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein sumoylation [IBA, ISO]
- protein ubiquitination [IBA, ISO]
- regulation of receptor activity [ISO]
Gene Ontology Molecular Function- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
Mus musculus
PREY
PIAS3
Pias3l
protein inhibitor of activated STAT 3
GO Process (4)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
PIAS3 induces SUMO-1 modification and transcriptional repression of IRF-1.
Interferon regulatory factor-1 (IRF-1) is a transcriptional activator that is involved in interferon response, regulation of cell growth and oncogenesis. To try to identify the molecules that regulate the function of IRF-1, we performed yeast two-hybrid screening and isolated protein inhibitor of activated STAT3 (PIAS3) as an IRF-1-binding protein. This protein was also found to bind with small ubiquitin-related modifier-1 ... [more]
FEBS Lett. Oct. 23, 2002; 530(1);204-8 [Pubmed: 12387893]
Throughput
- Low Throughput
Additional Notes
- Figure 2
Curated By
- BioGRID