BAIT

MDM2

1700007J15Rik, AA415488, Mdm-2
transformed mouse 3T3 cell double minute 2
GO Process (24)
GO Function (9)
GO Component (9)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Pax3 Stimulates p53 Ubiquitination and Degradation Independent of Transcription.

Wang XD, Morgan SC, Loeken MR

Pax3 is a developmental transcription factor that is required for neural tube and neural crest development. We previously showed that inactivating the p53 tumor suppressor protein prevents neural tube and cardiac neural crest defects in Pax3-mutant mouse embryos. This demonstrates that Pax3 regulates these processes by blocking p53 function. Here we investigated the mechanism by which Pax3 blocks p53 function.We ... [more]

PLoS ONE Jan. 05, 2012; 6(12);e29379 [Pubmed: 22216266]

Throughput

  • Low Throughput

Additional Notes

  • E2:Ubch5c

Curated By

  • BioGRID