BAIT
GRF2
14-3-3 PROTEIN G-BOX FACTOR14 OMEGA, 14-3-3OMEGA, F3F9.16, F3F9_16, GF14 OMEGA, general regulatory factor 2, AT1G78300
14-3-3-like protein GF14 omega
GO Process (1)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Arabidopsis thaliana (Columbia)
PREY
GI
FB, GIGANTEA, T22J18.6, T22J18_6, AT1G22770
protein GIGANTEA
GO Process (11)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- circadian rhythm [IDA]
- flower development [TAS]
- positive regulation of long-day photoperiodism, flowering [IMP]
- regulation of circadian rhythm [IMP]
- regulation of transcription, DNA-templated [IDA]
- response to blue light [IDA]
- response to cold [IMP]
- response to far red light [IMP]
- response to hydrogen peroxide [IMP]
- response to karrikin [IEP]
- temperature compensation of the circadian clock [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA, ISM]
Arabidopsis thaliana (Columbia)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana.
In eukaryotes, 14-3-3 dimers regulate hundreds of functionally diverse proteins (clients), typically in phosphorylation-dependent interactions. To uncover new clients, 14-3-3 omega (At1g78300) from Arabidopsis was engineered with a "tandem affinity purification" tag and expressed in transgenic plants. Purified complexes were analyzed by tandem MS. Results indicate that 14-3-3 omega can dimerize with at least 10 of the 12 14-3-3 isoforms ... [more]
Proteomics Jun. 01, 2009; 9(11);2967-85 [Pubmed: 19452453]
Throughput
- Low Throughput
Curated By
- BioGRID