An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Fluorescence lifetime imaging of interactions between Golgi tethering factors and small GTPases in plants.

Osterrieder A, Carvalho CM, Latijnhouwers M, Johansen JN, Stubbs C, Botchway S, Hawes C

Peripheral tethering factors bind to small GTPases in order to obtain their correct location within the Golgi apparatus. Using fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) we visualized interactions between Arabidopsis homologues of tethering factors and small GTPases at the Golgi stacks in planta. Co-expression of the coiled-coil proteins AtGRIP and golgin candidate 5 (GC5) [TATA ... [more]

Traffic Aug. 01, 2009; 10(8);1034-46 [Pubmed: 19490533]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ATGRIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

GC5