BAIT

UBC

2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

REV1

1110027I23Rik, AU022044, Rev1l
REV1 homolog (S. cerevisiae)
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Mus musculus

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage.

Guo C, Tang TS, Bienko M, Parker JL, Bielen AB, Sonoda E, Takeda S, Ulrich HD, Dikic I, Friedberg EC

REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of ... [more]

Mol. Cell. Biol. Dec. 01, 2006; 26(23);8892-900 [Pubmed: 16982685]

Throughput

  • Low Throughput

Additional Notes

  • #LPPI
  • Figure 1
  • Likely protein-protein interaction

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
REV1 UBC
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
1107253

Curated By

  • BioGRID