USP8
Gene Ontology Biological Process
- cell proliferation [TAS]
- endosome organization [IMP]
- mitotic cytokinesis [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RASGRF1
Gene Ontology Biological Process
- activation of Rac GTPase activity [IDA]
- cell proliferation [IMP]
- neuron projection development [IDA]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of Ras protein signal transduction [ISO]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [IGI]
- regulation of Rac protein signal transduction [IDA]
- regulation of Ras protein signal transduction [IDA]
- regulation of neuronal synaptic plasticity [IMP]
- regulation of synaptic plasticity [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [IDA]
- growth cone [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Cloning and characterization of mouse UBPy, a deubiquitinating enzyme that interacts with the ras guanine nucleotide exchange factor CDC25(Mm)/Ras-GRF1.
We used yeast "two-hybrid" screening to isolate cDNA-encoding proteins interacting with the N-terminal domain of the Ras nucleotide exchange factor CDC25(Mm). Three independent overlapping clones were isolated from a mouse embryo cDNA library. The full-length cDNA was cloned by RACE-polymerase chain reaction. It encodes a large protein (1080 amino acids) highly homologous to the human deubiquitinating enzyme hUBPy and contains ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 5B
Curated By
- BioGRID