Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Crystallization of small proteins assisted by green fluorescent protein.

Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, Dikic I, Drew D, Iwata S, Wakatsuki S, Kawasaki M

The generation of crystal lattice contacts by proteinaceous tags fused to target proteins is an attractive approach to aid in the crystallization of otherwise intractable proteins. Here, the use of green fluorescent protein (GFP) fusions for this purpose is demonstrated, using ubiquitin and the ubiquitin-binding motif (UBM) of Y-family polymerase Î¹ as examples. The structure of the GFP-ubiquitin fusion protein ... [more]

Acta Crystallogr. D Biol. Crystallogr. Oct. 01, 2010; 66(0);1059-66 [Pubmed: 20944239]

Throughput

Low Throughput

Additional Notes

#LPPI
GenBank accession No. X51703
Likely protein-protein interaction

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
POLI UBC	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Burschowsky D (2010)	Low	-	BioGRID	672148
POLI UBC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Burschowsky D (2010)	Low	-	BioGRID	672149

Curated By

BioGRID

Interaction Summary

UBC

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [ISO]protease binding [ISO]protein binding [IPI]

Gene Ontology Cellular Component

POLI