An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A calmodulin-binding site on cyclin E mediates Ca2+-sensitive G1/s transitions in vascular smooth muscle cells.

Choi J, Chiang A, Taulier N, Gros R, Pirani A, Husain M

Calcium transients are known to control several transition points in the eukaryotic cell cycle. For example, we have previously shown that a coordinate elevation in the intracellular free calcium ion concentration is required for G1- to S-phase cell cycle progression in vascular smooth muscle cells (VSMC). However, the molecular basis for this Ca2+ sensitivity was not known. Using buffers with ... [more]

Circ. Res. May. 26, 2006; 98(10);1273-81 [Pubmed: 16627785]

Throughput

Low Throughput

Additional Notes

Figure 6

Curated By

BioGRID

Interaction Summary

CCNE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [NAS]protein binding [IPI]transcription coactivator activity [NAS]

Gene Ontology Cellular Component

CALM1