CCND1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA, ISO]
- Leydig cell differentiation [ISO]
- Wnt signaling pathway [IDA]
- canonical Wnt signaling pathway [IMP]
- cellular response to DNA damage stimulus [ISO]
- cellular response to organic substance [IDA]
- endoplasmic reticulum unfolded protein response [IDA]
- fat cell differentiation [IDA]
- lactation [IGI, IMP]
- mammary gland alveolus development [IGI, IMP]
- mammary gland epithelial cell proliferation [IGI]
- mitotic G1 DNA damage checkpoint [ISO]
- negative regulation of Wnt signaling pathway [IMP]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of epithelial cell differentiation [IGI]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of G2/M transition of mitotic cell cycle [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity [ISO]
- positive regulation of mammary gland epithelial cell proliferation [IGI]
- positive regulation of protein phosphorylation [ISO]
- protein phosphorylation [IDA]
- re-entry into mitotic cell cycle [IDA]
- regulation of G1/S transition of mitotic cell cycle [IGI]
- regulation of cell cycle [IDA]
- regulation of protein kinase activity [IDA]
- response to UV-A [ISO]
- response to organonitrogen compound [ISO]
Gene Ontology Molecular Function- cyclin-dependent protein serine/threonine kinase regulator activity [IDA]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- kinase activity [IDA]
- proline-rich region binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [IPI, ISO]
- transcription corepressor activity [ISO]
- transcription factor binding [ISO]
- cyclin-dependent protein serine/threonine kinase regulator activity [IDA]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- kinase activity [IDA]
- proline-rich region binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [IPI, ISO]
- transcription corepressor activity [ISO]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
CDK4
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO]
- negative regulation of cell cycle arrest [ISO]
- positive regulation of G2/M transition of mitotic cell cycle [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of cell size [ISO]
- positive regulation of fibroblast proliferation [ISO]
- positive regulation of translation [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of cell cycle [IDA]
- regulation of cell proliferation [IMP]
- regulation of gene expression [ISO]
- response to drug [ISO]
- response to lead ion [ISO]
- response to organic substance [ISO]
- response to testosterone [ISO]
- signal transduction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
A calmodulin-binding site on cyclin E mediates Ca2+-sensitive G1/s transitions in vascular smooth muscle cells.
Calcium transients are known to control several transition points in the eukaryotic cell cycle. For example, we have previously shown that a coordinate elevation in the intracellular free calcium ion concentration is required for G1- to S-phase cell cycle progression in vascular smooth muscle cells (VSMC). However, the molecular basis for this Ca2+ sensitivity was not known. Using buffers with ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 1
- kinase assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CCND1 CDK4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 658152 | |
| CDK4 CCND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 658154 | |
| CDK4 CCND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CDK4 CCND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 658601 | |
| CDK4 CCND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CDK4 CCND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| CCND1 CDK4 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| CDK4 CCND1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID