BAIT
PARK2
PRKN
Parkinson disease (autosomal recessive, juvenile) 2, parkin
GO Process (48)
GO Function (21)
GO Component (21)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- aggresome assembly [ISO]
- cellular protein catabolic process [ISO]
- cellular response to toxic substance [ISO]
- dopamine metabolic process [IMP]
- dopamine uptake involved in synaptic transmission [IMP]
- learning [IMP]
- locomotory behavior [IMP]
- mitochondrion degradation [ISO]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO]
- negative regulation of glucokinase activity [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of neuron death [ISO]
- negative regulation of oxidative stress-induced neuron death [ISO]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of reactive oxygen species metabolic process [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- norepinephrine metabolic process [IMP]
- positive regulation of DNA binding [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of mitochondrial fusion [ISO]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- protein metabolic process [IMP]
- protein monoubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of autophagy [ISO]
- regulation of mitochondrion organization [ISO]
- regulation of neurotransmitter secretion [IMP]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [IMP]
- response to endoplasmic reticulum stress [ISO]
- response to unfolded protein [ISO]
- startle response [IMP]
- synaptic transmission, dopaminergic [IMP]
- synaptic transmission, glutamatergic [IMP]
Gene Ontology Molecular Function- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [ISO]
- LUBAC complex [ISO]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO]
- aggresome [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- intracellular [ISO]
- membrane raft [ISO]
- mitochondrion [ISO]
- neuron projection [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [ISO]
- protein complex [IDA]
- synapse [ISO]
- synaptic vesicle [ISO]
- ubiquitin ligase complex [ISO]
Mus musculus
PREY
UBE2L3
C79827, UbcM4, Ubce7
ubiquitin-conjugating enzyme E2L 3
GO Process (11)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- cell cycle phase transition [ISO]
- cell proliferation [ISO]
- cellular response to glucocorticoid stimulus [ISO]
- cellular response to steroid hormone stimulus [ISO]
- positive regulation of protein ubiquitination [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- protein K11-linked ubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Mus musculus
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
SH3 domains from a subset of BAR proteins define a Ubl-binding domain and implicate parkin in synaptic ubiquitination.
Mutations in the parkin gene are responsible for a common inherited form of Parkinson's disease (PD). Parkin is a RING-type E3 ubiquitin ligase with an N-terminal ubiquitin-like domain (Ubl). We report here that the parkin Ubl binds SH3 domains from endocytic BAR proteins such as endophilin-A with an affinity comparable to proline-rich domains (PRDs) from well-established SH3 partners. The NMR ... [more]
Mol. Cell Dec. 25, 2009; 36(6);1034-47 [Pubmed: 20064468]
Throughput
- Low Throughput
Additional Notes
- E2: UbcH7
- Parkin weakly ubiquitinates endopholin-A1 in vitro
Curated By
- BioGRID