Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction.

Tanaka S, Komeda Y, Umemori T, Kubota Y, Takisawa H, Araki H

Dpb11/Cut5/TopBP1 is evolutionarily conserved and is essential for the initiation of DNA replication in eukaryotes. The Dpb11 of the budding yeast Saccharomyces cerevisiae has four BRCT domains (BRCT1 to -4). The N-terminal pair (BRCT1 and -2) and the C-terminal pair (BRCT3 and -4) bind to cyclin-dependent kinase (CDK)-phosphorylated Sld3 and Sld2, respectively. These phosphorylation-dependent interactions trigger the initiation of DNA ... [more]

Mol. Cell. Biol. Jul. 01, 2013; 33(13);2614-22 [Pubmed: 23629628]

Throughput

Low Throughput

Additional Notes

Figure 5
requires co-expression of GINS2, GINS3, GINS4

Curated By

BioGRID

Interaction Summary

TOPBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

GINS1