BAIT

OZS1

CARBON DIOXIDE INSENSITIVE 3, CDI3, F5O11.23, F5O11_23, OZONE-SENSITIVE 1, RADICAL-INDUCED CELL DEATH 3, RCD3, SLAC1, SLOW ANION CHANNEL-ASSOCIATED 1, AT1G12480
guard cell S-type anion channel SLAC1
Arabidopsis thaliana (Columbia)
PREY

PP2CA

AHG3, ARABIDOPSIS THALIANA PROTEIN PHOSPHATASE 2CA, ATPP2CA, protein phosphatase 2CA, AT3G11410
protein phosphatase 2CA
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells.

Lee SC, Lan W, Buchanan BB, Luan S

The plant hormone abscisic acid (ABA) serves as a physiological monitor to assess the water status of plants and, under drought conditions, induces stomatal pore closure by activating specific ion channels, such as a slow-anion channel (SLAC1) that, in turn, mediate ion efflux from the guard cells. Earlier genetic analyses uncovered a protein kinase (OST1) and several 2C-type phosphatases, as ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 15, 2009; 106(50);21419-24 [Pubmed: 19955427]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PP2CA OZS1
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
1173100

Curated By

  • BioGRID