Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Selective homo- and heteromer interactions between the multiple organellar RNA editing factor (MORF) proteins in Arabidopsis thaliana.

Zehrmann A, Haertel B, Glass F, Bayer-Csaszar E, Obata T, Meyer E, Brennicke A, Takenaka M

RNA editing in plastids and mitochondria of flowering plants requires pentatricopeptide repeat proteins (PPR proteins) for site recognition and proteins of the multiple organellar RNA editing factor (MORF) family as cofactors. Two MORF proteins, MORF5 and MORF8, are dual-targeted to plastids and mitochondria; two are targeted to plastids, and five are targeted to mitochondria. Pulldown assays from Arabidopsis thaliana tissue ... [more]

J. Biol. Chem. Mar. 06, 2015; 290(10);6445-56 [Pubmed: 25583991]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT4G20020 AT5G44780	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Zehrmann A (2015)	Low	-	BioGRID	-
AT5G44780 AT4G20020	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zehrmann A (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT4G20020

Gene Ontology Biological Process

Gene Ontology Cellular Component

AT5G44780