BAIT

LAPTM5

E3, RP23-412B2.5
lysosomal-associated protein transmembrane 5
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Mus musculus
PREY

NEDD4

AA959633, AL023035, AU019897, E430025J12Rik, Nedd4-1, Nedd4a, mKIAA0093
neural precursor cell expressed, developmentally down-regulated 4
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization.

Fouladkou F, Landry T, Kawabe H, Neeb A, Lu C, Brose N, Stambolic V, Rotin D

PTEN is a tumor suppressor frequently mutated in cancer. Recent reports implicated Nedd4-1 as the E3 ubiquitin ligase for PTEN that regulates its stability and nuclear localization. We tested the physiological role of Nedd4-1 as a PTEN regulator by using cells and tissues derived from two independently generated strains of mice with their Nedd4-1 gene disrupted. PTEN stability and ubiquitination ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 24, 2008; 105(25);8585-90 [Pubmed: 18562292]

Throughput

  • Low Throughput

Curated By

  • BioGRID