Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

Burz DS, Dutta K, Cowburn D, Shekhtman A

We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural ... [more]

Nat. Methods Feb. 01, 2006; 3(2);91-3 [Pubmed: 16432517]

Throughput

Low Throughput

Additional Notes

#LPPI
Likely protein-protein interaction
NMR
This experiment was done in vivo in bacterial cells.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBC STAM2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	McLelland GL (2018)	Low	-	BioGRID	2513476
UBC STAM2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhang X (2017)	High	-	BioGRID	2297218

Curated By

BioGRID

Interaction Summary

STAM2

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

UBC

Gene Ontology Biological Process