BAIT

IAA29

F10M6.1, indole-3-acetic acid inducible 29, AT4G32280
auxin-responsive protein IAA29
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

NPH4

ARF7, AUXIN RESPONSE FACTOR 7, AUXIN-REGULATED TRANSCRIPTIONAL ACTIVATOR 7, AUXIN-RESPONSIVE TRANSCRIPTIONAL ACTIVATOR 7, BIP, BIPOSTO, IAA21, IAA23, IAA25, MASSUGU 1, MSG1, NON-PHOTOTROPHIC HYPOCOTYL, T1M15.130, T1M15_130, TIR5, TRANSPORT INHIBITOR RESPONSE 5, indole-3-acetic acid inducible 21, indole-3-acetic acid inducible 23, indole-3-acetic acid inducible 25, AT5G20730
auxin-regulated transcriptional activator NPH4
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The auxin signalling network translates dynamic input into robust patterning at the shoot apex.

Vernoux T, Brunoud G, Farcot E, Morin V, Van den Daele H, Legrand J, Oliva M, Das P, Larrieu A, Wells D, Guedon Y, Armitage L, Picard F, Guyomarc'h S, Cellier C, Parry G, Koumproglou R, Doonan JH, Estelle M, Godin C, Kepinski S, Bennett M, De Veylder L, Traas J

The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin ... [more]

Mol. Syst. Biol. Jul. 08, 2011; 7(0);508 [Pubmed: 21734647]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IAA29 NPH4
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
NPH4 IAA29
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
IAA29 NPH4
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID