A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

A membrane protein/signaling protein interaction network for Arabidopsis version AMPv2.

Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA, Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y, Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM, Schroeder JI, Rhee SY, Frommer WB

Interactions between membrane proteins and the soluble fraction are essential for signal transduction and for regulating nutrient transport. To gain insights into the membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list of 8,383 representing membrane and signaling proteins from Arabidopsis thaliana were cloned into a Gateway-compatible vector. The mating-based split ubiquitin system was used to screen ... [more]

Front Physiol Jan. 01, 2010; 1(0);24 [Pubmed: 21423366]

Throughput

High Throughput

Additional Notes

split-ubiquitin screen

Curated By

BioGRID

Interaction Summary

RGA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA, ISS, TAS]transcription regulatory region DNA binding [IPI]

Gene Ontology Cellular Component

TMKL1