An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate.

Sawasaki T, Kamura N, Matsunaga S, Saeki M, Tsuchimochi M, Morishita R, Endo Y

Protein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis. Here, we report that the Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate. Furthermore, we show three applications using the microarray: (1) ... [more]

FEBS Lett. Jan. 23, 2008; 582(2);221-8 [Pubmed: 18082144]

Throughput

Low Throughput

Additional Notes

Figure 3
autophosphorylation

Curated By

BioGRID

Interaction Summary

PAK3

Gene Ontology Biological Process

Gene Ontology Molecular Function

MAP kinase kinase activity [IDA]protein binding [IPI]receptor signaling protein serine/threonine kinase activity [IBA]

Gene Ontology Cellular Component