BAIT

CAMK2D

CAMKD
calcium/calmodulin-dependent protein kinase II delta
GO Process (32)
GO Function (8)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens
PREY

CAMK2D

CAMKD
calcium/calmodulin-dependent protein kinase II delta
GO Process (32)
GO Function (8)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate.

Sawasaki T, Kamura N, Matsunaga S, Saeki M, Tsuchimochi M, Morishita R, Endo Y

Protein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis. Here, we report that the Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate. Furthermore, we show three applications using the microarray: (1) ... [more]

FEBS Lett. Jan. 23, 2008; 582(2);221-8 [Pubmed: 18082144]

Throughput

  • Low Throughput

Additional Notes

  • Figure 3
  • autophosphorylation

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CAMK2D CAMK2D
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
CAMK2D CAMK2D
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID