BAIT
SK14
ASK14, SKP1-like 14, T18E12.16, T18E12_16, AT2G03170
SKP1-like protein 14
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
PREY
ZTL
ADAGIO 1, ADO1, FKF1-LIKE PROTEIN 2, FKL2, LKP1, LOV KELCH PROTEIN 1, MSF19.2, MSF19_2, ZEITLUPE, AT5G57360
adagio protein 1
GO Process (8)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IC]
- circadian rhythm [IMP, ISS, TAS]
- entrainment of circadian clock by photoperiod [IMP]
- flower development [IMP]
- proteasomal protein catabolic process [IMP]
- regulation of circadian rhythm [IMP]
- response to red light [IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A high-throughput system for two-hybrid screening based on growth curve analysis in microtiter plates.
The yeast two-hybrid system is a powerful tool for identifying novel protein-protein interactions. In general, biochemical marker genes such as lacZ are exploited for indirect quantification of the interaction, and commonly involve the conduct of rather laborious beta-galactosidase assays. This paper describes a simple alternative method based on growth curve analysis of yeast cultures that is amenable to microtiter plate ... [more]
Anal. Biochem. May. 15, 2003; 316(2);171-4 [Pubmed: 12711337]
Throughput
- High Throughput
Curated By
- BioGRID