BAIT

S6K2

ARABIDOPSIS THALIANA PROTEIN KINASE 2, ARABIDOPSIS THALIANA SERINE/THREONINE PROTEIN KINASE 2, ATPK19, ATPK2, ATS6K2, Arabidopsis thaliana protein kinase 19, serine/threonine protein kinase 2, AT3G08720
serine/threonine protein kinase 2
GO Process (5)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

TAP46

2A phosphatase associated protein of 46 kD, MNB8.6, MNB8_6, AT5G53000
PP2A regulatory subunit TAP46
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Overexpression of the PP2A regulatory subunit Tap46 leads to enhanced plant growth through stimulation of the TOR signalling pathway.

Ahn CS, Ahn HK, Pai HS

Tap46, a regulatory subunit of protein phosphatase 2A (PP2A), plays an essential role in plant growth and development through a functional link with the Target of Rapamycin (TOR) signalling pathway. Here, we have characterized the molecular mechanisms behind a gain-of-function phenotype of Tap46 and its relationship with TOR to gain further insights into Tap46 function in plants. Constitutive overexpression of ... [more]

J. Exp. Bot. Feb. 01, 2015; 66(3);827-40 [Pubmed: 25399018]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAP46 S6K2
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
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Curated By

  • BioGRID