PIP3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SYP121
Gene Ontology Biological Process
- defense response [TAS]
- defense response to fungus [IGI]
- intracellular protein transport [TAS]
- jasmonic acid mediated signaling pathway [IGI]
- maintenance of protein location in plasma membrane [IMP]
- membrane fusion [TAS]
- negative regulation of cellular defense response [IGI]
- negative regulation of defense response [IMP]
- negative regulation of programmed cell death [IGI]
- protein targeting to membrane [IDA]
- protein targeting to plasma membrane [IMP]
- regulation of plant-type hypersensitive response [IGI]
- regulation of stomatal movement [IMP]
- response to abscisic acid [IEP]
- response to fungus [IMP]
- salicylic acid mediated signaling pathway [IGI]
- transpiration [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Arabidopsis SNAREs SYP61 and SYP121 coordinate the trafficking of plasma membrane aquaporin PIP2;7 to modulate the cell membrane water permeability.
Plant plasma membrane intrinsic proteins (PIPs) are aquaporins that facilitate the passive movement of water and small neutral solutes through biological membranes. Here, we report that post-Golgi trafficking of PIP2;7 in Arabidopsis thaliana involves specific interactions with two syntaxin proteins, namely, the Qc-SNARE SYP61 and the Qa-SNARE SYP121, that the proper delivery of PIP2;7 to the plasma membrane depends on ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PIP3 SYP121 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID