BAIT
CMR1
YDL156W
DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
SAC3
LEP1, L000001792, YDR159W
mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
GO Process (9)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- actin filament-based process [IGI]
- mRNA 3'-end processing [IMP]
- mRNA export from nucleus [IGI, IMP, IPI]
- mitotic nuclear division [IMP]
- nuclear retention of pre-mRNA at the site of transcription [IMP]
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
- protein export from nucleus [IGI, IMP]
- ribosomal small subunit biogenesis [IMP]
- transcription-coupled nucleotide-excision repair [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control.
DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1-together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins-define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated ... [more]
Nat Commun Mar. 31, 2015; 6(0);6533 [Pubmed: 25817432]
Quantitative Score
- 6.8 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- SGA performed with cmr1 as bait in the presence of hydroxyurea; cut-off is 0.25 for positive genetic and 1.50 for negative genetic
Curated By
- BioGRID