BAIT

AT1G10820

T16B5.4, T16B5_4
hypothetical protein
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AT5G58900

K19M22.10, K19M22_10
homeodomain-like transcriptional regulator
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interplay of MYB factors in differential cell expansion, and consequences for tomato fruit development.

Machemer K, Shaiman O, Salts Y, Shabtai S, Sobolev I, Belausov E, Grotewold E, Barg R

We previously identified SlFSM1 as an early fruit-specific gene encoding a short protein harboring a non-canonical SANT/MYB-like domain. Here, we investigated the role of FSM1 during fruit development in tomato and its mode of action. By analyzing tomato plants ectopically expressing FSM1, we established that it negatively affects cell expansion, particularly of those cells with the highest potential to expand, ... [more]

Plant J. Oct. 01, 2011; 68(2);337-50 [Pubmed: 21707804]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT1G10820 AT5G58900
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID