BAIT

ABI1

ABA INSENSITIVE 1, AtABI1, F20B18.190, F20B18_190, PROTEIN PHOSPHATASE 2C ABI1, AT4G26080
protein phosphatase 2C 56
Arabidopsis thaliana (Columbia)
PREY

SNRK2.3

SNRK2-3, SRK2I, SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2-3, sucrose nonfermenting 1(SNF1)-related protein kinase 2.3, AT5G66880
serine/threonine-protein kinase SRK2I
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases.

Soon FF, Ng LM, Zhou XE, West GM, Kovach A, Tan MH, Suino-Powell KM, He Y, Xu Y, Chalmers MJ, Brunzelle JS, Zhang H, Yang H, Jiang H, Li J, Yong EL, Cutler S, Zhu JK, Griffin PR, Melcher K, Xu HE

Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. At the center of the ABA signaling network is a subfamily of type 2C protein phosphatases (PP2Cs), which form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase (SnRK2s). Here, we report a SnRK2-PP2C complex structure, which reveals marked similarity in PP2C recognition by SnRK2 and ... [more]

Science Jan. 06, 2012; 335(6064);85-8 [Pubmed: 22116026]

Throughput

  • Low Throughput

Curated By

  • BioGRID