BAIT

MYB69

ATMYB69, F17M5.210, F17M5_210, MYB DOMAIN PROTEIN 69, AT4G33450
myb domain protein 69
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

BPM1

ATBPM1, BTB-POZ AND MATH DOMAIN 1, T16G12.40, T16G12_40, AT5G19000
BTB-POZ and math domain-containing protein
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of Arabidopsis MYB56 as a novel substrate for CRL3(BPM) E3 ligases.

Chen L, Bernhardt A, Lee J, Hellmann H

Controlled stability of proteins is a highly efficient mechanism to direct diverse processes in living cells. A key regulatory system for protein stability is given by the ubiquitin proteasome pathway, which uses E3 ligases to mark specific proteins for degradation. In this work, MYB56 is identified as a novel target of a CULLIN3 (CUL3)-based E3 ligase. Its stability depends on the ... [more]

Mol Plant Feb. 01, 2015; 8(2);242-50 [Pubmed: 25618823]

Throughput

  • Low Throughput

Curated By

  • BioGRID