BAIT

TCP3

F12M16.13, F12M16_13, TEOSINTE BRANCHED 1, cycloidea and PCF transcription factor 3, AT1G53230
TEOSINTE BRANCHED 1, cycloidea and PCF transcription factor 3
Arabidopsis thaliana (Columbia)
PREY

WRKY28

ATWRKY28, T9A21.10, T9A21_10, WRKY DNA-binding protein 28, AT4G18170
WRKY DNA-binding protein 28
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

TCP transcription factors are critical for the coordinated regulation of isochorismate synthase 1 expression in Arabidopsis thaliana.

Wang X, Gao J, Zhu Z, Dong X, Wang X, Ren G, Zhou X, Kuai B

Salicylic acid (SA) plays an important role in various aspects of plant development and responses to stresses. To elucidate the sophisticated regulatory mechanism of SA synthesis and signaling, we used a yeast one-hybrid system to screen for regulators of isochorismate synthase 1 (ICS1), a gene encoding the key enzyme in SA biosynthesis in Arabidopsis thaliana. A TCP family transcription factor ... [more]

Plant J. Apr. 01, 2015; 82(1);151-62 [Pubmed: 25702611]

Throughput

  • Low Throughput

Additional Notes

  • BiFC

Curated By

  • BioGRID