PREY

AUX1

AUXIN RESISTANT 1, F16M14.5, F16M14_5, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, PIR1, WAV5, WAVY ROOTS 5, AT2G38120
auxin transporter protein 1
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity.

Yu H, Moss BL, Jang SS, Prigge M, Klavins E, Nemhauser JL, Estelle M

The phytohormone auxin regulates virtually every aspect of plant development. The hormone directly mediates the interaction between the two members of the auxin coreceptor complex, a TRANSPORT INHIBITOR RESPONSE (TIR1)/AUXIN SIGNALING F-BOX protein and an AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressor. To learn more about the interaction between these proteins, a mutant screen was performed using the yeast (Saccharomyces cerevisiae) two-hybrid ... [more]

Plant Physiol. May. 01, 2013; 162(1);295-303 [Pubmed: 23539280]

Throughput

  • Low Throughput

Curated By

  • BioGRID