BAIT

PDV1

K19E1.8, K19E1_8, PLASTID DIVISION1, AT5G53280
plastid division protein PDV1
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ARC5

ACCUMULATION AND REPLICATION OF CHLOROPLAST 5, DRP5B, Dynamin related protein 5B, AT3G19720
dynamin-like protein ARC5
GO Process (3)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Plastid division control: the PDV proteins regulate DRP5B dynamin activity.

Holtsmark I, Lee S, Lunde KA, Auestad K, Maple-Grodem J, Moller SG

Chloroplast division represents a fundamental but complex biological process involving remnants of the ancestral bacterial division machinery and proteins of eukaryotic origin. Moreover, the chloroplast division machinery is divided into stromal and cytosolic sub machineries, which coordinate and control their activities to ensure appropriate division initiation and progression. Dynamin related protein 5B (DRP5B) and plastid division protein 1 and 2 ... [more]

Plant Mol. Biol. Jun. 01, 2013; 82(3);255-66 [Pubmed: 23595201]

Throughput

  • Low Throughput

Additional Notes

  • Figure 3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARC5 PDV1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
1115350

Curated By

  • BioGRID