An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The RNA helicases AtMTR4 and HEN2 target specific subsets of nuclear transcripts for degradation by the nuclear exosome in Arabidopsis thaliana.

Lange H, Zuber H, Sement FM, Chicher J, Kuhn L, Hammann P, Brunaud V, Berard C, Bouteiller N, Balzergue S, Aubourg S, Martin-Magniette ML, Vaucheret H, Gagliardi D

The RNA exosome is the major 3'-5' RNA degradation machine of eukaryotic cells and participates in processing, surveillance and turnover of both nuclear and cytoplasmic RNA. In both yeast and human, all nuclear functions of the exosome require the RNA helicase MTR4. We show that the Arabidopsis core exosome can associate with two related RNA helicases, AtMTR4 and HEN2. Reciprocal ... [more]

PLoS Genet. Aug. 01, 2014; 10(8);e1004564 [Pubmed: 25144737]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT3G07750 RRP41	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	McWhite CD (2020)	High	0.9895	BioGRID	2910870
AT3G07750 RRP41	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Braun P (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RRP41

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5'-exoribonuclease activity [ISS]RNA binding [ISS]

Gene Ontology Cellular Component

AT3G07750