HEN1
Gene Ontology Biological Process
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- production of miRNAs involved in gene silencing by miRNA [IMP, NAS]
- production of siRNA involved in RNA interference [IGI, IMP]
- regulation of flower development [IMP]
- specification of floral organ identity [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
HYL1
Gene Ontology Biological Process
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- pre-miRNA processing [IMP]
- production of miRNAs involved in gene silencing by miRNA [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- response to abscisic acid [IMP]
- response to auxin [IMP]
- response to cytokinin [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins.
Methylation of 3'-terminal nucleotides of miRNA/miRNA* is part of miRNAs biogenesis in plants but is not found in animals. In Arabidopsis thaliana this reaction is carried out by a multidomain AdoMet-dependent 2'-O-methyltransferase HEN1. Using deletion and structure-guided mutational analysis, we show that the double-stranded RNA-binding domains R(1) and R(2) of HEN1 make significant but uneven contributions to substrate RNA binding, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HYL1 HEN1 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| HYL1 HEN1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID