DCL1
Gene Ontology Biological Process
- RNA processing [ISS]
- cytokinesis by cell plate formation [IMP]
- embryonic pattern specification [IMP]
- flower development [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- primary miRNA processing [TAS]
- production of lsiRNA involved in RNA interference [IMP]
- production of miRNAs involved in gene silencing by miRNA [IGI]
- production of siRNA involved in RNA interference [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of seed maturation [IMP]
- seed morphogenesis [IMP]
- suspensor development [IMP]
- vegetative to reproductive phase transition of meristem [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HEN1
Gene Ontology Biological Process
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- production of miRNAs involved in gene silencing by miRNA [IMP, NAS]
- production of siRNA involved in RNA interference [IGI, IMP]
- regulation of flower development [IMP]
- specification of floral organ identity [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins.
Methylation of 3'-terminal nucleotides of miRNA/miRNA* is part of miRNAs biogenesis in plants but is not found in animals. In Arabidopsis thaliana this reaction is carried out by a multidomain AdoMet-dependent 2'-O-methyltransferase HEN1. Using deletion and structure-guided mutational analysis, we show that the double-stranded RNA-binding domains R(1) and R(2) of HEN1 make significant but uneven contributions to substrate RNA binding, ... [more]
Throughput
- Low Throughput
Related interactions
Curated By
- BioGRID