BAIT

JAZ10

JAS1, JASMONATE-ASSOCIATED 1, T31B5.40, T31B5_40, TIFY 9, TIFY DOMAIN PROTEIN 9, TIFY9, jasmonate-zim-domain protein 10, AT5G13220
protein TIFY 9
Arabidopsis thaliana (Columbia)
PREY

AT1G01260

F6F3.7, F6F3_7
transcription factor bHLH13
GO Process (1)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The bHLH subgroup IIId factors negatively regulate jasmonate-mediated plant defense and development.

Song S, Qi T, Fan M, Zhang X, Gao H, Huang H, Wu D, Guo H, Xie D

Plants have evolved sophisticated systems for adaptation to their natural habitat. In response to developmental and environmental cues, plants produce and perceive jasmonate (JA) signals, which induce degradation of JASMONATE-ZIM-Domain (JAZ) proteins and derepress the JAZ-repressed transcription factors to regulate diverse aspects of defense responses and developmental processes. Here, we identified the bHLH subgroup IIId transcription factors (bHLH3, bHLH13, bHLH14 ... [more]

PLoS Genet. Aug. 13, 2013; 9(7);e1003653 [Pubmed: 23935516]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT1G01260 JAZ10
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
AT1G01260 JAZ10
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
JAZ10 AT1G01260
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID