An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interplay of Fli-I and FLAP1 for regulation of beta-catenin dependent transcription.

Lee YH, Stallcup MR

Beta-catenin mediates Wnt/wingless signaling and transcriptional activation by lymphocyte enhancer binding factor 1/T cell factor (LEF1/TCF) proteins with the assistance of multiple coregulators, including positive cofactors like p300/CBP and negative cofactors like HDACs. We previously demonstrated that a developmentally essential protein, Flightless-I (Fli-I), serves as a coactivator for nuclear receptor-mediated transcription. To further understand the action mechanism of Fli-I, we ... [more]

Nucleic Acids Res. Sep. 23, 2006; 34(18);5052-9 [Pubmed: 16990252]

Throughput

Low Throughput

Additional Notes

figure 3A.

Curated By

BioGRID

Interaction Summary

GRIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [NAS]beta-catenin binding [TAS]glucocorticoid receptor binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]receptor signaling complex scaffold activity [NAS]transcription coactivator activity [NAS]

Gene Ontology Cellular Component

FLII