RNF8
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IDA, ISO]
- double-strand break repair [ISO]
- double-strand break repair via nonhomologous end joining [IMP]
- histone H2A K63-linked ubiquitination [ISO]
- histone H2A ubiquitination [IMP, ISO]
- histone H2B ubiquitination [IMP]
- histone exchange [IMP]
- isotype switching [IMP]
- negative regulation of transcription elongation from RNA polymerase II promoter [ISO]
- positive regulation of DNA repair [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- response to ionizing radiation [ISO]
- spermatid development [IMP]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE2N
Gene Ontology Biological Process
- DNA double-strand break processing [ISO]
- T cell receptor signaling pathway [ISO, ISS]
- double-strand break repair via homologous recombination [ISO]
- histone ubiquitination [ISO]
- positive regulation of DNA repair [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO, ISS]
- positive regulation of histone modification [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- postreplication repair [IBA, ISO]
- protein K63-linked ubiquitination [IBA, ISO]
- protein ubiquitination [ISO]
- regulation of histone ubiquitination [ISO]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chfr and RNF8 synergistically regulate ATM activation.
Protein ubiquitination is a crucial component of the DNA damage response. To study the mechanism of the DNA damage-induced ubiquitination pathway, we analyzed the impact of the loss of two E3 ubiquitin ligases, RNF8 and Chfr. Notably, DNA damage-induced activation of ATM kinase is suppressed in cells deficient in both RNF8 and Chfr (double-knockout, or DKO), and DKO mice develop ... [more]
Throughput
- Low Throughput
Additional Notes
- figure S4a.
Curated By
- BioGRID