CHFR
Gene Ontology Biological Process
- mitotic cell cycle [IMP]
- mitotic cell cycle checkpoint [ISO]
- modification-dependent protein catabolic process [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- protein destabilization [IDA]
- protein polyubiquitination [IDA, ISO]
- protein ubiquitination [IMP]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
UBE2V1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chfr and RNF8 synergistically regulate ATM activation.
Protein ubiquitination is a crucial component of the DNA damage response. To study the mechanism of the DNA damage-induced ubiquitination pathway, we analyzed the impact of the loss of two E3 ubiquitin ligases, RNF8 and Chfr. Notably, DNA damage-induced activation of ATM kinase is suppressed in cells deficient in both RNF8 and Chfr (double-knockout, or DKO), and DKO mice develop ... [more]
Throughput
- Low Throughput
Additional Notes
- figure S4a.
Curated By
- BioGRID