SPA1
Gene Ontology Biological Process
- chloroplast organization [IMP]
- negative regulation of photomorphogenesis [IMP]
- photomorphogenesis [IMP]
- red or far-red light signaling pathway [IMP]
- red, far-red light phototransduction [IGI]
- regulation of photoperiodism, flowering [IMP]
- response to far red light [IEP]
- response to red light [IEP]
- short-day photoperiodism, flowering [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CRY2
Gene Ontology Biological Process
- chromatin organization [IMP]
- chromatin remodeling [IMP]
- circadian regulation of calcium ion oscillation [IMP]
- phototropism [IGI]
- positive regulation of flower development [IMP]
- regulation of flower development [IDA]
- regulation of meristem growth [IGI]
- response to blue light [IMP]
- response to light stimulus [IEP]
- response to water deprivation [IGI]
- stomatal movement [IGI]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism.
Cryptochromes (CRYs) are blue-light photoreceptors that mediate various light responses in plants and animals. The signaling mechanism by which CRYs regulate light responses involves their physical interactions with COP1. Here, we report that CRY1 interacts physically with SPA1 in a blue-light-dependent manner. SPA acts genetically downstream from CRYs to regulate light-controlled development. Blue-light activation of CRY1 attenuates the association of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRY2 SPA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CRY2 SPA1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CRY2 SPA1 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | - | |
| CRY2 SPA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CRY2 SPA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID