CREBBP
Gene Ontology Biological Process
- N-terminal peptidyl-lysine acetylation [ISO]
- cell proliferation [ISO]
- germ-line stem cell maintenance [IMP]
- histone acetylation [IDA, ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
Gene Ontology Cellular Component
RPS6KA3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mitogen-regulated RSK2-CBP interaction controls their kinase and acetylase activities.
The protein kinase ribosomal S6 kinase 2 (RSK2) has been implicated in phosphorylation of transcription factor CREB and histone H3 in response to mitogenic stimulation by epidermal growth factor. Binding of phospho-CREB to the coactivator CBP allows gene activation through recruitment of the basal transcriptional machinery. Acetylation of H3 by histone acetyltransferase (HAT) activities, such as the one carried by ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 1B, right
Curated By
- BioGRID