BAIT

SUN1

ARABIDOPSIS SAD1/UNC-84 DOMAIN PROTEIN 1, ATSUN1, MUG13.15, MUG13_15, SAD1/UNC-84 domain protein 1, AT5G04990
SAD1/UNC-84 domain protein 1
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

WIP2

K24C1.2, K24C1_2, WPP domain interacting protein 2, AT5G56210
WPP domain-interacting protein 2
GO Process (1)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The kinesin AtPSS1 promotes synapsis and is required for proper crossover distribution in meiosis.

Duroc Y, Lemhemdi A, Larcheveque C, Hurel A, Cuacos M, Cromer L, Horlow C, Armstrong SJ, Chelysheva L, Mercier R

Meiotic crossovers (COs) shape genetic diversity by mixing homologous chromosomes at each generation. CO distribution is a highly regulated process. CO assurance forces the occurrence of at least one obligatory CO per chromosome pair, CO homeostasis smoothes out the number of COs when faced with variation in precursor number and CO interference keeps multiple COs away from each other along ... [more]

PLoS Genet. Oct. 01, 2014; 10(10);e1004674 [Pubmed: 25330379]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SUN1 WIP2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
WIP2 SUN1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID