An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis.

Fujiwara S, Sakamoto S, Kigoshi K, Suzuki K, Ohme-Takagi M

Biological functions of only some plant transcriptional repressors are known owing to the lack of knockout lines or unclear phenotypes because of redundancy. Here we show that strong viral activation domain VP16 fusion to the transcriptional repressor FLOWERING LOCUS C reversed its function and caused a stronger phenotype than that of the multiple-knockout line of redundant genes, suggesting the potential ... [more]

FEBS Lett. Oct. 16, 2014; 588(20);3665-72 [Pubmed: 25150167]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FLC PFT1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Fujiwara S (2014)	Low	-	BioGRID	1172837

Curated By

BioGRID

Interaction Summary

FLC

Gene Ontology Biological Process

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [IDA, ISS, TAS]

Gene Ontology Cellular Component

PFT1