BAIT

SFR6

SENSITIVE TO FREEZING 6, T1J1.2, T1J1_2, AT4G04920
mediator of RNA polymerase II transcription subunit 16
Arabidopsis thaliana (Columbia)
PREY

FRU

ARABIDOPSIS FE-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1, ATBHLH029, ATBHLH29, ATFIT1, BASIC HELIX-LOOP-HELIX PROTEIN 29, BHLH029, F24D13.5, F24D13_5, FE-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1, FER-like regulator of iron uptake, FIT1, AT2G28160
FER-LIKE IRON DEFICIENCY-INDUCED transcription factor
Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis.

Zhang Y, Wu H, Wang N, Fan H, Chen C, Cui Y, Liu H, Ling HQ

Iron is an essential nutrient for plant growth and development, and its absorption is tightly controlled. Under iron limitation, FIT dimerizes with the four Ib bHLH proteins and activates the expression of iron uptake genes. However, how the dimerized complex activates downstream genes remains unclear. Using forward genetics, a low-iron-sensitive mutant was screened. The corresponding gene (MED16) was isolated, and ... [more]

New Phytol. Aug. 01, 2014; 203(3);770-83 [Pubmed: 24889527]

Throughput

  • Low Throughput

Additional Notes

  • BiFC assay

Curated By

  • BioGRID