BAIT

PIF4

MFL8.13, MFL8_13, SRL2, phytochrome interacting factor 4, AT2G43010
transcription factor PIF4
Arabidopsis thaliana (Columbia)
PREY

ARF6

T4K22.6, T4K22_6, auxin response factor 6, AT1G30330
auxin response factor 6
GO Process (3)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl.

Oh E, Zhu JY, Bai MY, Arenhart RA, Sun Y, Wang ZY

As the major mechanism of plant growth and morphogenesis, cell elongation is controlled by many hormonal and environmental signals. How these signals are coordinated at the molecular level to ensure coherent cellular responses remains unclear. Here, we illustrate a molecular circuit that integrates all major growth-regulating signals, including auxin, brassinosteroid, gibberellin, light, and temperature. Analyses of genome-wide targets, genetic and ... [more]

Elife May. 27, 2014; 0(0);e03031 [Pubmed: 24867218]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PIF4 ARF6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID