BAIT

GRI

F8L10.2, F8L10_2, GRIM REAPER, AT1G53130
protein GRIM REAPER
Arabidopsis thaliana (Columbia)
PREY

AT1G50610

F11F12.7, F11F12_7
probably inactive leucine-rich repeat receptor-like protein kinase
GO Process (3)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis.

Wrzaczek M, Vainonen JP, Stael S, Tsiatsiani L, Help-Rinta-Rahko H, Gauthier A, Kaufholdt D, Bollhoener B, Lamminmaeki A, Staes A, Gevaert K, Tuominen H, Van Breusegem F, Helariutta Y, Kangasjaervi J

Recognition of extracellular peptides by plasma membrane-localized receptor proteins is commonly used in signal transduction. In plants, very little is known about how extracellular peptides are processed and activated in order to allow recognition by receptors. Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI) is dependent on the activity of ... [more]

EMBO J. Jan. 02, 2015; 34(1);55-66 [Pubmed: 25398910]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GRI AT1G50610
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Low-BioGRID
-

Curated By

  • BioGRID